Human Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from human brain tissue. Human Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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Jeu 8 Fév 2018 - 7:46