HCoEpiC are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescent method with antibodies to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 10 population doublings in the conditions provided by Creative Bioarray.