2d dige service for biological research

2d dige service for biological research

Images of different channel represent different protein samples. Superimpose of these images reveal the difference between different protein samples. For example, if protein A is upregulated, the intensity of the signal of the spot, which corresponds to protein A, will be much higher. When a internal standard protein is also run in the same gel, the absolute amount of proteins contained in each sample can be calculated based on the signal intensity. In addition to protein quantification study, DIGE can also be applied to study protein post-translational modifications. The types of modification can be reflected by the shift of proteins on the gel image.